Rüter, Axel; Kuczera, Stefan; Pochan, Darrin J.; Olsson, Ulf
Twisted Ribbon Aggregates in a Model Peptide System
The model peptides A8K and A10K self-assemble in water into ca. 100 nm long ribbon-like aggregates. These structures can be described as ?-sheets laminated into a ribbon structure with a constant elliptical cross-section of 4 by 8 nm, where the longer axis corresponds to a finite number, N ? 15,…
Arguments for an additional long-lived intermediate in the photocycle of the full-length aureochrome 1c receptor: A time-resolved small-angle X-ray scattering study
Bannister, Saskia; Böhm, Elena; Zinn, Thomas; Hellweg, Thomas; Kottke, Tilman
Aureochromes (AUREO) act as blue-light photoreceptors in algae. They consist of a light-, oxygen-, voltage-sensitive (LOV) domain and a DNA-binding basic region/leucine zipper. Illumination of the flavin cofactor in LOV leads to the formation of an adduct, followed by global structural changes. Here, we first applied UV/vis spectroscopy to characterize…
NMR and crystallographic structural studies of the extremely stable monomeric variant of human cystatin C with single amino acid substitution
Maszota‐Zieleniak, Martyna; Jurczak, Przemyslaw; Orlikowska, Marta; Zhukov, Igor; Borek, Dominika; Otwinowski, Zbyszek; Skowron, Piotr; Pietralik, Zuzanna; Kozak, Maciej; Szymańska, Aneta; Rodziewicz‐Motowidło, Sylwia
Human cystatin C (hCC), a member of the superfamily of papain-like cysteine protease inhibitors, is the most widespread cystatin in human body fluids. This small protein, in addition to its physiological function, is involved in various diseases, including cerebral amyloid angiopathy, cerebral hemorrhage, stroke, and dementia. Physiologically active hCC is…
PrP (58–93) peptide from unstructured N-terminal domain of human prion protein forms amyloid-like fibrillar structures in the presence of Zn2+ ions
Gielnik, Maciej; Pietralik, Zuzanna; Zhukov, Igor; Szymańska, Aneta; Kwiatek, Wojciech M.; Kozak, Maciej
Many transition metal ions modulate the aggregation of different amyloid peptides. Substoichiometric zinc concentrations can inhibit aggregation, while an excess of zinc can accelerate the formation of cytotoxic fibrils. In this study, we report the fibrillization of the octarepeat domain to amyloid-like structures. Interestingly, this self-assembling process occurred only in…
The domain swapping of human cystatin C induced by synchrotron radiation
Taube, Michal; Pietralik, Zuzanna; Szymanska, Aneta; Szutkowski, Kosma; Clemens, Daniel; Grubb, Anders; Kozak, Maciej
Domain swapping is observed for many proteins with flexible conformations. This phenomenon is often associated with the development of conformational diseases. Importantly, domain swapping has been observed for human cystatin C (HCC), a protein capable of forming amyloid deposits in brain arteries. In this study, the ability of short exposure…
Alginate-coating of artemisinin-loaded cochleates results in better control over gastro-intestinal release for effective oral delivery
Shuddhodana; Judeh, Zaher
Development of new drug delivery systems for effective delivery of poorly water-soluble drugs is important. We report the development of cochleate-based formulation for the oral administration of poorly water-soluble artemisinin (ART). Cochleates offer great potential as vehicles for the oral delivery of ART due to their high drug encapsulation and…
Molecular architecture of the Jumonji C family histone demethylase KDM5B
Jerzy Dorosz, Line Hyltoft Kristensen, Nanda G. Aduri, Osman Mirza, Rikke Lousen, Saskia Bucciarelli, Ved Mehta, Selene Sellés-Baiget, Sara Marie Øie Solbak, Anders Bach, Pablo Mesa, Pablo Alcon Hernandez, Guillermo Montoya, Tam T. T. N. Nguyen, Kasper D. Rand, Thomas Boesen & Michael Gajhede
The full length human histone 3 lysine 4 demethylase KDM5B (PLU-1/Jarid1B) has been studied using Hydrogen/Deuterium exchange mass spectrometry, homology modelling, sequence analysis, small angle X-ray scattering and electron microscopy ...
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument
S. Bucciarelli, S. R. Midtgaard, M. Nors Pedersen, S. Skou, L. Arleth and B. Vestergaard
Coupling of size-exclusion chromatography with biological solution small-angle X-ray scattering (SEC-SAXS) on dedicated synchrotron beamlines enables structural analysis of challenging samples such as labile proteins and low-affinity complexes...
Temperature-Induced Self-Assembly of the Group B Streptococcus (GBS) Fusion Antigen GBS-NN
Rose, Fabrice; Roovers, Silke; Fano, Mathias; Harloff-Helleberg, Stine; Kirkensgaard, Jacob J.K.; Hejnaes, Kim; Fischer, Per; Foged, Camilla
Group B Streptococcus (GBS) is a leading cause of serious bacterial neonatal infections worldwide, which provides an unmet medical need for a globally effective vaccine. The recombinant GBS fusion antigen GBS-NN contains the N-terminal regions of the GBS Rib and Alpha C proteins. It shows promising immunogenicity eliciting protective immunity…
Size-Selective Adsorption in Nanoporous Polymers from Coumarin Photo-Cross-Linked Columnar Liquid Crystals
Concellón, Alberto; Schenning, Albertus P. H. J.; Romero, Pilar; Marcos, Mercedes; Serrano, José Luis
We have prepared nanoporous polymers from columnar hexagonal hydrogen-bonded complexes, whose order is fixed by coumarin photoinduced cycloaddition (photodimerization). Two different hydrogen-bonded complexes were used and consisted of a melamine (M) or tris(triazolyl)triazine (T) derivative acting as central templates and three peripheral carboxylic acids containing coumarin units. These…