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Structural biology

Temperature-Induced Self-Assembly of the Group B Streptococcus (GBS) Fusion Antigen GBS-NN

Rose, Fabrice; Roovers, Silke; Fano, Mathias; Harloff-Helleberg, Stine; Kirkensgaard, Jacob J.K.; Hejnaes, Kim; Fischer, Per; Foged, Camilla

 ApplicationCharacterizationCustomer publicationsMaterialParticle size and size distributionProteins & DNA/RNASAXSSelf-assembled materialStructural biologyTechniqueTemperature

Temperature-Induced Self-Assembly of the Group B Streptococcus (GBS) Fusion Antigen GBS-NN

Rose, Fabrice; Roovers, Silke; Fano, Mathias; Harloff-Helleberg, Stine; Kirkensgaard, Jacob J.K.; Hejnaes, Kim; Fischer, Per; Foged, Camilla

Group B Streptococcus (GBS) is a leading cause of serious bacterial neonatal infections worldwide, which provides an unmet medical need for a globally effective vaccine. The recombinant GBS fusion antigen GBS-NN contains the N-terminal regions of the GBS Rib and Alpha C proteins. It shows promising immunogenicity eliciting protective immunity…
Phosphorylation-induced conformational dynamics in an intrinsically disordered protein and potential role in phenotypic heterogeneity

Kulkarni, Prakash; Jolly, Mohit Kumar; Jia, Dongya; Mooney, Steven M.; Bhargava, Ajay; Kagohara, Luciane T.; Chen, Yihong; Hao, Pengyu; He, Yanan; Veltri, Robert W.; Grishaev, Alexander; Weninger, Keith; Levine, Herbert; Orban, John

 ApplicationCharacterizationCustomer publicationsMaterialProteins & DNA/RNASAXSShapeStructural biologyTechnique

Phosphorylation-induced conformational dynamics in an intrinsically disordered protein and potential role in phenotypic heterogeneity

Kulkarni, Prakash; Jolly, Mohit Kumar; Jia, Dongya; Mooney, Steven M.; Bhargava, Ajay; Kagohara, Luciane T.; Chen, Yihong; Hao, Pengyu; He, Yanan; Veltri, Robert W.; Grishaev, Alexander; Weninger, Keith; Levine, Herbert; Orban, John

Intrinsically disordered proteins (IDPs) that lack a unique 3D structure and comprise a large fraction of the human proteome play important roles in numerous cellular functions. Prostate-Associated Gene 4 (PAGE4) is an IDP that acts as a potentiator of the Activator Protein-1 (AP-1) transcription factor. Homeodomain-Interacting Protein Kinase 1 (HIPK1)…
Effects of oxidation on the physicochemical properties of polyunsaturated lipid membranes

Parra-Ortiz, Elisa; Browning, Kathryn L.; Damgaard, Liv S. E.; Nordström, Randi; Micciulla, Samantha; Bucciarelli, Saskia; Malmsten, Martin

 ApplicationCharacteristic dimensionCharacterizationCustomer publicationsMaterialPhase analysisSAXSSelf-assembled materialStructural biologyTechnique

Effects of oxidation on the physicochemical properties of polyunsaturated lipid membranes

Parra-Ortiz, Elisa; Browning, Kathryn L.; Damgaard, Liv S. E.; Nordström, Randi; Micciulla, Samantha; Bucciarelli, Saskia; Malmsten, Martin

The exposure of biological membranes to reactive oxygen species (ROS) plays an important role in many pathological conditions such as inflammation, infection, or sepsis. ROS also modulate signaling processes and produce markers for damaged tissue. Lipid peroxidation, mainly affecting polyunsaturated phospholipids, results in a complex mixture of oxidized products, which…
Disruptive effect of tocopherol oxalate on DPPC liposome structure: DSC, SAXS, and fluorescence anisotropy studies

Neunert, Grażyna; Tomaszewska-Gras, Jolanta; Siejak, Przemyslaw; Pietralik, Zuzanna; Kozak, Maciej; Polewski, Krzysztof

 Characteristic dimensionCustomer publicationsLipidsSAXSStructural biology

Disruptive effect of tocopherol oxalate on DPPC liposome structure: DSC, SAXS, and fluorescence anisotropy studies

Neunert, Grażyna; Tomaszewska-Gras, Jolanta; Siejak, Przemyslaw; Pietralik, Zuzanna; Kozak, Maciej; Polewski, Krzysztof

α-Tocopherol oxalate (TO), a tocopherol ester derivative, was investigated for its effect on the structural changes of fully hydrated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes, as a function of concentration and temperature, by applying differential scanning calorimetry (DSC), small angle X-ray scattering (SAXS), and DPH fluorescence anisotropy methods. The DSC and DPH…
Structural analysis of mtEXO mitochondrial RNA degradosome reveals tight coupling of nuclease and helicase components

Razew, Michal; Warkocki, Zbigniew; Taube, Michal; Kolondra, Adam; Czarnocki-Cieciura, Mariusz; Nowak, Elzbieta; Labedzka-Dmoch, Karolina; Kawinska, Aleksandra; Piatkowski, Jakub; Golik, Pawel; Kozak, Maciej; Dziembowski, Andrzej; Nowotny, Marcin

 Customer publicationsProteins & DNA/RNASAXSShapeStructural biology

Structural analysis of mtEXO mitochondrial RNA degradosome reveals tight coupling of nuclease and helicase components

Razew, Michal; Warkocki, Zbigniew; Taube, Michal; Kolondra, Adam; Czarnocki-Cieciura, Mariusz; Nowak, Elzbieta; Labedzka-Dmoch, Karolina; Kawinska, Aleksandra; Piatkowski, Jakub; Golik, Pawel; Kozak, Maciej; Dziembowski, Andrzej; Nowotny, Marcin

The mitochondrial RNA degradosome (mtEXO) plays an essential role in the regulation of mitochondrial gene expression and is composed of the 3′-to-5′ exoribonuclease Dss1 and the helicase Suv3. Here the authors present the RNA bound mtEXO crystal structure and give insights into its…
Rheological Link Between Polymer Melts with a High Molecular Weight Tail and Enhanced Formation of Shish-Kebabs

Wingstrand, Sara L.; Shen, Bo; Kornfield, Julie A.; Mortensen, Kell; Parisi, Daniele; Vlassopoulos, Dimitris; Hassager, Ole

 CharacterizationCustomer publicationsLipidsProteins & DNA/RNASAXSStructural biology

Rheological Link Between Polymer Melts with a High Molecular Weight Tail and Enhanced Formation of Shish-Kebabs

Wingstrand, Sara L.; Shen, Bo; Kornfield, Julie A.; Mortensen, Kell; Parisi, Daniele; Vlassopoulos, Dimitris; Hassager, Ole

Presence of an ultra high molecular weight (UHMw) fraction in flowing polymer melts is known to facilitate formation of oriented crystalline structures significantly. The UHMw fraction manifests itself as a minor tail in the molar mass distribution and is hardly detectable in the canonical characterization methods. In this study, alternatively,…
Immobilization of the enzyme invertase in SBA-15 with surfaces functionalized by different organic compounds

Ribeiro, Livia M. O.; Falleiros, Larissa N. S. S.; de Resende, Miriam M.; Ribeiro, Eloízio J.; Almeida, Renata M. R. G.; da Silva, Antonio O. S.

 Customer publicationsPhase analysisPorous materialSAXSStructural biology

Immobilization of the enzyme invertase in SBA-15 with surfaces functionalized by different organic compounds

Ribeiro, Livia M. O.; Falleiros, Larissa N. S. S.; de Resende, Miriam M.; Ribeiro, Eloízio J.; Almeida, Renata M. R. G.; da Silva, Antonio O. S.

The search for appropriate supports for enzyme immobilization has attracted increasing interest in the field of biocatalysis. Immobilized enzymes are catalysts of enormous industrial interest because they combine the advantages of heterogeneous catalysis with the high selectivity and mild operational conditions of enzymes. This study evaluated the use of different…
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