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Structural biology

Distinguishing Between Monomeric scFv and Diabody in Solution Using Light and Small Angle X-ray Scattering

Lüdel, Frank; Bufe, Sandra; Bleymüller, Willem M.; de Jonge, Hugo; Iamele, Luisa; Niemann, Hartmut H.; Hellweg, Thomas

 ApplicationCharacterizationCustomer publicationsMaterialOtherProteins & DNA/RNASAXSShapeStructural biologyTechnique

Distinguishing Between Monomeric scFv and Diabody in Solution Using Light and Small Angle X-ray Scattering

Lüdel, Frank; Bufe, Sandra; Bleymüller, Willem M.; de Jonge, Hugo; Iamele, Luisa; Niemann, Hartmut H.; Hellweg, Thomas

Depending on the linker length between the V H and the V L domain, single-chain Fv (scFv) antibody fragments form monomers, dimers (diabodies) or higher oligomers. We aimed at generating a diabody of the anti-MET antibody 3H3 to use it as crystallization chaperone to promote crystallization of the MET ectodomain…
Improvement of Antialveolar Echinococcosis Efficacy of Albendazole by a Novel Nanocrystalline Formulation with Enhanced Oral Bioavailability

Hu, Chunhui; Liu, Zhengsheng; Liu, Chengyu; Zhang, Yaogang; Fan, Haining; Qian, Feng

 (Natural) polymersApplicationCharacteristic dimensionCharacterizationCustomer publicationsMaterialSAXSStructural biologyTechnique

Improvement of Antialveolar Echinococcosis Efficacy of Albendazole by a Novel Nanocrystalline Formulation with Enhanced Oral Bioavailability

Hu, Chunhui; Liu, Zhengsheng; Liu, Chengyu; Zhang, Yaogang; Fan, Haining; Qian, Feng

Alveolar echinococcosis (AE) is a chronic infectious parasitic disease that is fatal and still being neglected. Currently, the AE treatment recommended by the WHO is complete excision of the lesions, followed by the oral administration of albendazole (ABZ), the only effective first-line anti-AE drug, for two years. Unfortunately, complete excision…
Characterization of phospholipid vesicles containing lauric acid: physicochemical basis for process and product development

Farkuh, Laura; Hennies, Paulo T.; Nunes, Cláudia; Reis, Salette; Barreiros, Luisa; Segundo, Marcela A.; Oseliero Filho, Pedro L.; Oliveira, Cristiano L. P.; Cassago, Alexandre; Portugal, Rodrigo V.; Muramoto, Rodrigo A.; Carretero, Gustavo P. B.; Schreier, Shirley; Chaimovich, Hernan; Cuccovia, Iolanda M.

 ApplicationCharacterizationCustomer publicationsLipidsMaterialParticle interactionsParticlesPhase analysisSAXSSizeStructural biologySurfactantsTechnique

Characterization of phospholipid vesicles containing lauric acid: physicochemical basis for process and product development

Farkuh, Laura; Hennies, Paulo T.; Nunes, Cláudia; Reis, Salette; Barreiros, Luisa; Segundo, Marcela A.; Oseliero Filho, Pedro L.; Oliveira, Cristiano L. P.; Cassago, Alexandre; Portugal, Rodrigo V.; Muramoto, Rodrigo A.; Carretero, Gustavo P. B.; Schreier, Shirley; Chaimovich, Hernan; Cuccovia, Iolanda M.

Lauric acid (LAH) strongly inhibits the growth of acne-causing bacteria. LAH is essentially water-insoluble and the solubility of laurate (LA) salts are medium and temperature dependent. Hence, LAH/LA preparations are difficult to formulate. Here we fully characterized phospholipid vesicles containing up to 50 mol% LAH. Vesicles of dipalmitoylphosphatidylcholine (DPPC) containing…
Continuous, high-throughput production of artemisinin-loaded supramolecular cochleates using simple off-the-shelf flow focusing device

Shuddhodana; Wong, Pooi Wen Kathy; Judeh, Zaher

 ApplicationCharacteristic dimensionCharacterizationCustomer publicationsLipidsMaterialParticlesSAXSStructural biologyTechnique

Continuous, high-throughput production of artemisinin-loaded supramolecular cochleates using simple off-the-shelf flow focusing device

Shuddhodana; Wong, Pooi Wen Kathy; Judeh, Zaher

Lipid cochleates are gaining increasing interest as drug-carriers. However, their preparation relies on conventional batch processes that are complex, time consuming and lack batch-to-batch reproducibility; presenting a bottleneck for clinical translation. We report an efficient continuous preparation process for artemisinin-loaded cochleates (ART-cochleates) using inexpensive off-the-shelf flow…
Antigenic and physicochemical characterization of Hepatitis B surface protein under extreme temperature and pH conditions

Lopes, J. L. S.; Oliveira, D. C. A.; Utescher, C. L. A.; Quintilio, W.; Tenório, E. C. N.; Oliveira, C. L. P.; Fantini, M. C. A.; Rasmussen, M. K.; Bordallo, H. N.; Sant’Anna, O. A.; Botosso, V. F.

 ApplicationCharacterizationCustomer publicationsMaterialOtherProteins & DNA/RNASAXSShapeSizeStructural biologyTechnique

Antigenic and physicochemical characterization of Hepatitis B surface protein under extreme temperature and pH conditions

Lopes, J. L. S.; Oliveira, D. C. A.; Utescher, C. L. A.; Quintilio, W.; Tenório, E. C. N.; Oliveira, C. L. P.; Fantini, M. C. A.; Rasmussen, M. K.; Bordallo, H. N.; Sant’Anna, O. A.; Botosso, V. F.

Hepatitis B virus causes acute and chronic infections in millions of people worldwide and, since 1982, a vaccine with 95% effectiveness has been available for immunization. The main component of the recombinant hepatitis B vaccine is the surface antigen protein (HBsAg). In this work, the effect of pH, ionic strength…
Crystal Chemistry and SAXS Studies of an Octahedral Polyoxoarsenotungstate Nanocluster Encapsulating Four Unprecedented Thorium Arsenate Fragments (Th3As2On – n = 25 or 26)

Dufaye, Maxime; Duval, Sylvain; Stoclet, Grégory; Loiseau, Thierry

 ApplicationCharacteristic dimensionCharacterizationCustomer publicationsFractal dimensionMaterialPhase analysisPorous materialSAXSStructural biologyTechnique

Crystal Chemistry and SAXS Studies of an Octahedral Polyoxoarsenotungstate Nanocluster Encapsulating Four Unprecedented Thorium Arsenate Fragments (Th3As2On – n = 25 or 26)

Dufaye, Maxime; Duval, Sylvain; Stoclet, Grégory; Loiseau, Thierry

The reaction of 8– with the 5f tetravalent thorium cation in presence of an acetate buffer and KCl at pH = 4.7 gives rise to the formation of an octahedral polyoxoarsenotungstate nanocluster 38–. This molecule is constructed around four unprecedented Th3As2On (n = 25 or 26) fragments stabilized by the…
Nanoscopic Structure of Complexes Formed between DNA and the Cell-Penetrating Peptide Penetratin

de Mello, Lucas Rodrigues; Hamley, Ian William; Castelletto, Valeria; Garcia, Bianca Bonetto Moreno; Han, Sang Won; de Oliveira, Cristiano Luis Pinto; da Silva, Emerson Rodrigo

 ApplicationCharacterizationCustomer publicationsFractal dimensionMaterialProteins & DNA/RNASAXSSizeStructural biologyTechnique

Nanoscopic Structure of Complexes Formed between DNA and the Cell-Penetrating Peptide Penetratin

de Mello, Lucas Rodrigues; Hamley, Ian William; Castelletto, Valeria; Garcia, Bianca Bonetto Moreno; Han, Sang Won; de Oliveira, Cristiano Luis Pinto; da Silva, Emerson Rodrigo

One of the most remarkable examples of cell-penetrating peptides (CPPs) is Penetratin, a 16-mer fragment derived from the Drosophila Antennapedia homeobox. Understanding the structure of Penetratin/DNA complexes is a key factor for the successful design of new vectors for gene delivery and may assist in optimizing molecular carriers based on…
Proinflammatory Action of a New Electronegative Low-Density Lipoprotein Epitope

Faulin, Tanize do Espirito Santo; Kazuma, Soraya Megumi; Tripodi, Gustavo Luis; Cavalcante, Marcela Frota; Wakasuqui, Felipe; Oliveira, Cristiano Luis Pinto; Degenhardt, Maximilia Frazão de Souza; Michaloski, Jussara; Giordano, Ricardo José; Ketelhuth, Daniel Francisco Jacon; Abdalla, Dulcineia Saes Parra

 ApplicationCharacterizationCustomer publicationsMaterialOtherProteins & DNA/RNASAXSShapeStructural biologyTechnique

Proinflammatory Action of a New Electronegative Low-Density Lipoprotein Epitope

Faulin, Tanize do Espirito Santo; Kazuma, Soraya Megumi; Tripodi, Gustavo Luis; Cavalcante, Marcela Frota; Wakasuqui, Felipe; Oliveira, Cristiano Luis Pinto; Degenhardt, Maximilia Frazão de Souza; Michaloski, Jussara; Giordano, Ricardo José; Ketelhuth, Daniel Francisco Jacon; Abdalla, Dulcineia Saes Parra

The electronegative low-density lipoprotein, LDL (?), is an endogenously modified LDL subfraction with cytotoxic and proinflammatory actions on endothelial cells, monocytes, and macrophages contributing to the progression of atherosclerosis. In this study, epitopes of LDL (?) were mapped using a phage display library of peptides and monoclonal antibodies reactive to…
The influence of pH, protein concentration and calcium ratio on the formation and structure of nanotubes from partially hydrolyzed bovine ?-lactalbumin

Geng, XiaoLu; Kirkensgaard, Jacob Judas Kain; Arleth, Lise; Otte, Jeanette; Ipsen, Richard

 ApplicationCharacterizationCustomer publicationsFoodFood scienceMaterialProteins & DNA/RNASAXSSelf-assembled materialShapeSizeStructural biologyTechnique

The influence of pH, protein concentration and calcium ratio on the formation and structure of nanotubes from partially hydrolyzed bovine ?-lactalbumin

Geng, XiaoLu; Kirkensgaard, Jacob Judas Kain; Arleth, Lise; Otte, Jeanette; Ipsen, Richard

Formation of nanotubes from partially hydrolysed ?-lactalbumin (?-La) was investigated at five pH values, two concentrations of ?-La and two calcium levels. Nanotubes were formed under almost all combinations of the investigated factors, and for the first time the formation of nanotubes at low pH (4.0) and low protein concentration…
X-ray Scattering and Coarse-Grained Simulations for Clustering and Interactions of Monoclonal Antibodies at High Concentrations

Dear, Barton J.; Bollinger, Jonathan A.; Chowdhury, Amjad; Hung, Jessica J.; Wilks, Logan R.; Karouta, Carl A.; Ramachandran, Kishan; Shay, Tony Y.; Nieto, Maria P.; Sharma, Ayush; Cheung, Jason K.; Nykypanchuk, Dmytro; Godfrin, P. Douglas; Johnston, Keith P.; Truskett, Thomas M.

 ApplicationCharacterizationCustomer publicationsMaterialParticle interactionsProteins & DNA/RNASAXSStructural biologyTechnique

X-ray Scattering and Coarse-Grained Simulations for Clustering and Interactions of Monoclonal Antibodies at High Concentrations

Dear, Barton J.; Bollinger, Jonathan A.; Chowdhury, Amjad; Hung, Jessica J.; Wilks, Logan R.; Karouta, Carl A.; Ramachandran, Kishan; Shay, Tony Y.; Nieto, Maria P.; Sharma, Ayush; Cheung, Jason K.; Nykypanchuk, Dmytro; Godfrin, P. Douglas; Johnston, Keith P.; Truskett, Thomas M.

Attractive protein–protein interactions (PPI) in concentrated monoclonal antibody (mAb) solutions may lead to reversible oligomers (clusters) that impact colloidal stability and viscosity. Herein, the PPI are tuned for two mAbs via the addition of arginine (Arg), NaCl, or ZnSO4 as characterized by the structure factor (Seff(q)) with small-angle X-ray scattering…
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