Measurements and analysis of extremely thin single protein fibers
Silks are well known natural protein fibers, spun by spiders, silkworms and other insects, that have been used for centuries in the textile industry due to...
Characterization of protein self-association
Bovine serum albumin (BSA) is a non-glycosylated globular protein found in bovines. It is widely used in drug delivery, immunodiagnostic procedures and in clinical chemistry due to its low cost and...
Fast thermal studies of cocoa butter
Cocoa butter is a highly polymorphic compound exhibiting up to six crystalline forms of glycerides (fatty acids), each defined by different stability and melting point. In order to...
Batch data analysis of particle size temporal evolution studied with SAXS
Time-resolved Small Angle X-ray Scattering (SAXS) mesurements, performed during synthesis procedures or other chemical or physical reactions, have the potential to reveal valuable information which can...
Number concentrations for Gold Nanoparticles: robust and traceable measurements in the lab
SAXS laboratory instruments provide traceable number concentration measurements for nanoparticles. Nanoparticles (NPs) are an essential building block for ...
Size distribution of nanoparticles: powders, dispersions and composites
Size distributions of industrial nanoparticles have been determined in various forms for quality assessment and process monitoring. Nanoparticles are being produced at an increasing rate and have become an essential building block for the development of new materials with improved properties. The performance of the final products depends not only…
Vesicles
Probing the effect of oxidation on model lipid bilayers The vital function of cells depends strongly on cell membranes which enclose cellular compartments and separate the cell interior from the extracellular domain. Oxidative stress for example damages the cell membrane and is at the origin of many pathological conditions such…
Microgels
Screening of stimuli-responsive colloidal particles Microgels are colloidal particles consisting of cross-linked 3-dimensional polymer networks. These particles have the ability to shrink or swell substantially in response to external stimuli such as temperature, pH, ionic strength and light, allowing triggered release of encapsulated bioactive molecules. They additionally protect these loaded…
Thorough investigation of oligomers during protein fibrillation
The transient nature of aggregates during the fibrillation process of α-synuclein is highlighted with in-house SAXS experiments using the BioXolver. Protein aggregation into fibrillar structures is associated with a variety of neurodegenerative disorders, such as Parkinson’s and Alzheimer’s diseases. It is becoming increasingly evident that the toxic species causing the…
In-lab SEC-SAXS for structural investigation of complex samples
How to study the structure of complex biological samples? High quality data and 3D envelopes can be obtained on small and large proteins with SEC-SAXS in the lab Small-angle X-ray scattering (SAXS) is commonly used to investigate the solution structure of biological macromolecules. Highly accurate results can be obtained with…